About Trypan Blue Cell Counting Majority of experimental procedure in cell cultures, such as transfections, cryopreservation, cell fusion techniques and subculture routines, it is necessary to count the cell number prior to use. Using a consistent number of cells will help to maintain optimum growth of cells and also help
About Cell Plating In order to culture cells for standardization of an experiment such as drug dosage, cytotoxicity, transfection or cell migration assays etc., a fairly good number of cells are required. In such a case cells are cultured on plates having wells. There are 6/ 24/ 96 well plates
What is Cell Migration? Cell migration, movement of cells from one area to another generally in response to a chemical stimulus. Migration is a central to achieving functions such as wound repair, cell differentiation and embryonic development, immune response, and disease processes such as cancer metastasis and inflammation. Methods to
What is Cell Culture? Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favourable artificial environment. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from
About Immunocytochemistry Immunocytochemistry (ICC) is a common laboratory technique that uses antibodies that target specific peptides or protein antigens in the cell via specific epitopes. The antibodies bound on diffirent epitotoes can then be detected using several different methods. Immunocytochemistry enables researchers to evaluate whether or not cells in a
About Calcein AM / PI Cell Viability Assays The Calcein –AM/PI assay is a two-colour fluorescence cell death assay that is based on the simultaneous determination of live and dead cells with two different probes. It measure recognized parameters of cell viability by evaluating intracellular esterase activity and plasma membrane
What is Electroporation? The process of introducing nucleic acids (DNA or RNA) into eukaryotic cells by nonviral methods is defined as transfection. Using various chemical, phospho-lipid or physical methods, this gene transfer technology is a powerful tool to study gene function and protein expression. Transfection is a method that neutralizes
About Apoptosis Annexin V is a phospholipid binding protein with high affinity for binding to phosphotidyl serine (PS), which is a phospholipid present exclusively in the inner leaflet of the cell membrane. Annexin V does not bind to normal cells because of its inability to penetrate the lipid bilayer. During
About Neutral Red Cell Viability Assay The NRU cytotoxicity assay or Neutral red uptake assay procedure is a cell survival or cell viability chemosensitivity assay based on the ability of live cells to incorporate and bind neutral red (NR), a supravital dye. Principle of Neutral Red Cell Viability Assay NR
Gel Electrophoresis Objective To perform SDS-PAGE Gel Electrophoresis for separation of proteins. About Gel Electrophoresis Almost most of the analytical electrophoresis of proteins is carried out in polyacrylamide gels under conditions (generally SDS-PAGE) that ensure dissociation of the proteins into their individual polypeptide subunits. Most common anionic detergent SDS (Sodium