XXX XXX
Department of xxxx and xxxxx, xxxx
xxx, xxxx, xxx-xxx, India.
Email Id: xxxxxx@gmail.com, Tel.: (xxx) xxxx, Mobile: (+91) xxxxx
Current position: Women Scientist-A (DST) under the mentorship of Dr. xxxx, xxxx- xxxx, India
Educational details:
Ph.D. (Life Sciences), xxx-xxx
xxx, xxxx, India
Title of the Thesis: xxxxxxxxxxxxxxx
Registered on xxxxx at xxxx University, under the mentorship of Dr. xxxx, Assistant Director, xxxxx xxx xxxxx, India
M.Sc. (xxxx), xxxx-xxxx
xxxx, xxx University, xxxx, India
B.Sc. (Zoology, Chemistry, Botany), xxx-xxxx
xxxx, University xxxx, xxxx, India.
Publications:
Awards and Honors:
Research experience:
Research interests:
Techniques known and applied:
Abstract/ Poster presentations:
Personal details:
Father’s Name: Mr. xxx
Mother’s Name: Mrs. xxx
Sex: xxx
Date of Birth: xxxx
Nationality: Indian
Marital Status: xxxx
Languages: English, Hindi, German
Interests: listening to music, Playing Badminton. Reading Novels
Permanent Address: xxxx
References:
Assistant Director
Department of xxx
xxxx
xxx
Tel.: xxx
Director and Head
Department of xxxx,
xxxx
Tel.: xxxx, xxxx
I, hereby declare that the particulars furnished by me are true to the best of my knowledge and belief.
Place: xxx, India xxx xxx
XXXX
xxxx, Department of xxxx, xxx University xxxx
University of xxx, xxxx, xxx, United States of America
Email Id: xxxxxx@gmail.com, Mobile:+1-(xxx) xxx xxxx
Current position: Postdoctoral Research Associate in the lab of Dr. xxxx, xxx, USA.
Educational details:
Ph.D (Life Science)
Full intuitional address, India.
M.Sc (Biotechnology)
Full intuitional address, India.
B.Sc (Hons) (Zoology)
Full intuitional address, India.
Publications:
Manuscript communicated/under preparation:
Awards and Honors:
November xxx-current, Research Associate,
University of xxx, xxxx, United States of America.
Mentor: Dr. xxxx
Assistant Professor, Department of xxxx, University of xxx, USA.
Project title#1: “xxxxx”.
Summary of Project#1:
Phenotypic heterogeneity allows individual cells to behave different from the bulk. This non-genetic variability provides a critical route to therapeutic resistance and relapse of cancer. We identified rare, slow cycling, dynamic, pre-existing drug tolerant state in xxx-Cell xxxx. Single-cell analysis revealed a strong inherent drive to re-program daughter cells to occupy the xxxx state. Transcriptional reprogramming in resistant phenotype, in particular xxxxx helps in maintenance of an equilibrium between the drug-resistant and drug-sensitive cells. We further characterized, rare individual cells with the xxxx, xxx phenotype consistent with the identified profile of the drug-resistant state in biopsy lesions of xxxx cell xxx patients (manuscript under preparation).
Summary of Project#2:
Metabolic flexibility allows cells to adapt to various environments and limits efficacy of metabolic drugs. Therapeutic targeting of cancer metabolism relies on defining limiting requirements and vulnerabilities in the highly dynamic metabolic network of cancer cells. We characterized metabolic reprogramming in response to pharmacological inhibition of mitochondrial complex xxx in malignant xxxx lines, xenograft models, and isolated xxx cells. Metabolic adaptation to complex xxxx dysfunction is mediated by mitochondrial reactive oxygen species (mROS) serving as a stress signal that activates xxxx. Inhibition of the xxxx/xxxx axis selectively disrupts metabolic adaptation and survival during complex xxx dysfunction in malignant xxxx, xenograft models, acute lymphoblastic leukemia (ALL) and chronic lymphocytic leukemia (CLL) patient’s samples (manuscript under communication).
Summary of Project#3:
Emerging evidence indicates that cellular metabolism is a critical factor in cancer progression. We find that malignant xxx and xxx cells oscillate between non-invasive and highly invasive states providing a rationale for novel treatment approaches “locking” cells into a non-invasive state. The highly invasive state shows a reprogrammed metabolic profile with xxx xxx and xxx xxxx indicating a critical role of bioenergetic wiring in disease dissemination. Furthermore, the xxx invasive state shows xxx-dependent activation of xxx which is necessary for in vitro xxx and in vivo xxx of malignant cells. Interestingly, the metabolic reprogramming is independent of xxxx placing it in between metabolic xxx and xxx potential of xxx/xxxx cells (manuscript under communication).
December xxxx-May xxx, Ph.D,
xxx Institute, India.
Mentor: Dr. xxxxxx
Senior Scientist, xxxx, India.
Thesis title: “xxxxx”.
Summary of Doctoral work:
The paradoxical role of xxx in tumor immunology is widely accepted (xxxx et al; year, journal). However, contribution of xxxx in breast cancer progression and their molecular mechanism remains unexplored. Therefore, in my Ph.D thesis, I deciphered xxxx-mediated invasion, migration and molecular mechanism involved in breast cancer progression. I further, investigated xxxx regulation and their molecular signalling by xxxx in breast cancer cells. Furthermore, I investigated interplay of xxx and apoptosis induced by xxxx analogue (xxxx et al; year, journal). In addition, I also characterized signaling pathways of DNA damage and xxx induced by xxx analogue (xxxx et al; year, journal). On the other hand, I also worked on novel xxx agents in cancer cell death (xxxx et al; year, journal, xxxx et al; year, journal, xxxx et al; year, journal, xxxx et al; year, journal).
July xxxxx-August xxxx, M.Sc dissertation,
xxx University, India.
Mentor: Prof. xxxxx
Professor, xxxx, xxxx University, xxx, India.
Dissertation title: “xxxxxxx”.
Summary of Dissertation:
xxx vaccine lacks efficacy as well as lasting memory response and various other antigens failed to generate effective immune response. In my dissertation, we developed an xxx based vaccine that potentiates Th1 response against xxx infection and reduce xxxxx load in the animal model.
Working with interaction of xxxx and tumor, trigger immense interest in tumor microenvironment as well as tumor immunology. Complex network of immune cells infiltrations in tumor microenvironment, role of cytokine cross-talk in between, cytokine imbalance, cancer immunosurveillance, cancer cell dormancy, mechanism of relapse and their therapeutic benefits, and targeting of abnormal cell signaling and cell death pathways during cancer progression with novel and/or existing molecule fascinated me a lot.
Computer and other skills:
Oral talk/Poster presentations:
Personal details:
Father’s Name: xxxx
Mother’s Name: xxx
Sex: xxx
Date of Birth: xxx
Nationality: Indian
Marital Status: xxxx
Languages: English, Hindi and xxxx
Interests: Playing cricket, table tennis, singing and listening to music
Permanent Address: xxxx, India.
References:
Assistant Professor, Department of xxx, University of xxx, xxxxx, United States of America.
Email: xxxxx@gmail.com
Web-Page link: xxxxxxxx
Professor and Associate Vice Chair for Research, xxxx Research Laboratories, Department of xxxx, University xxxx, United States of America.
Email: xxxx@gmail.com
Web-Page link: xxxx
Senior Scientist, xxxx Research Institute, India.
Email: xxxx@gmail.com
Web-Page link: xxxxxx
Declaration
I, hereby declare that the particulars furnished by me are true to the best of my knowledge and belief.
Place: United States of America xxxxxxx
Name: xxxx
Full institutional address: xxx, xxxx
xxx, xx, xxx, India.
Email: xxxxx@gmail.com, Mobile: +91- xxx, xxxx, Skype id: xxx, xxxx
SUMMARY OF QUALIFICATIONS
Ph.D. in xxx: have studied the physical and functional connection of the entire proteome associated to xxx during transcription cycle using in vivo complex purification. I have implemented two different strategies (xxx tag and xxx tag) to investigate the distribution of transcription complexes and expect to get new proteins with yet unassigned function. Furthermore, I have identified the binding specificity of these protein or protein complex with respect to the phosphorylation pattern of xxxx in vitro and in vivo. Understanding the extent of gene regulation at the epigenetic level would help address and control the abnormal cellular behavior in cancer and other diseases and will eventually contribute towards more targeted treatment strategies.
Ad hoc Faculty: worked as Ad hoc faculty member (August-xxx to August-xxx) at Department of xxx Science in xxx University, xxx, INDIA. There, I was actively involved in departmental teaching, training and experimental program of B.Sc. and M.Sc. Students.
Post Doctoral Fellowship: Qualified and have successfully completed National-Post Doctoral Fellowship (N-PDF) (Title: *********** *********** *********** *********** *********** *********** *********** ***********) conducted by Science and Engineering Research Board (SERB- Grant no-PDF/ ***********) Department of Science and Technology, Government of India in ( xxx-xxxx).
Research Associate: Qualified and presently pursuing Research Associateship on project since xxx xxx (Title: *********** *********** *********** *********** *********** *********** *********** ***********) under the mentorship of Dr xxx xxx (Principal Scientist) xxx, xxxx India.
EDUCATION
Ph.D in xxx (Specialized in Molecular Biology, Microbiology, Proteomics, Immunology, Cell Biology)
Degree affiliated with xxx University, India
Research work done at xxxx Institute, India.
M.Sc (xxx)
In xxx with xxxx%, xxxx University, India.
B.Sc (xxx, xxx, xxx)
In xxx with xxx%, xxxx University, India.
PUBLICATIONS
MANUSCRIPTS UNDER REVIEW/COMMUNICATED/UNDER PREPARATION
*********** *********** *********** *********** *********** *********** *********** ***********
BOOK CHAPTERS PUBLISHED
*********** *********** *********** *********** *********** *********** *********** ***********
RESEARCH / TEACHING EXPERIENCE
Research Associate: Qualified and presently pursuing Research Associateship on project since xxx xxx (*********** *********** *********** *********** *********** *********** *********** ***********) under the mentorship of Dr xxx (Principal Scientist) xxxx, India.
Post Doctoral Fellowship: Qualified for and successfully completed (*********** *********** *********** *********** *********** *********** *********** ***********) National-Post Doctoral Fellowship (N-PDF) conducted by Science and Engineering Research Board (SERB- Grant no-PDF/*********** *********** *********** *********** *********** *********** *********** ***********) Department of Science and Technology, Government of India in xxxx xxxx.
Ad hoc Faculty: worked as Ad hoc faculty member (August-xxx to August-xxxx) at Department of xxxxx in xxxx University, INDIA. There, I was actively involved in departmental teaching, training and experimental program of B.Sc. and M.Sc. Students.
Doctrate Degree: January xxxx – July xxx, Ph.D at xxx Institute, India.
Project title: *********** *********** *********** *********** *********** *********** *********** ***********. Mentor: Dr. xxxxx
Objectives of the study:
*********** *********** *********** *********** *********** *********** *********** ***********Outcomes of the study:
M.Sc: August xxxx – July xxx, M.Sc dissertation at xxx University, India.
Dissertation title: A Study On The Cross reactivity Of xxxx Antibodies And xxxx Acquired From Various Plant Sources. Mentor: Dr. xxxx.
Objectives of the study:
Outcomes of the study:
SKILL / EXPERTISE
Biochemistry & Instrumentation: Protein concentration determination (Lowry, Bradford, UV-visible Method), Gel filtration chromatography (AKTA-FPLC), ion-exchange, Hydrophobic interaction chromatography (HIC), Affinity chromatography (His tag, GST and TAP tag purification), TLC (Thin Layer Chromatography), Ammonium sulfate precipitation, Protein Dialysis, Sonication, Ultra-centrifugation technique, Handling of various microscopes, Labeling (FITC and Alexa-488) of protein and its localization studies, Protein–protein interaction, Isothermal Titration Calorimetry (ITC), Surface Plasmon resonance (SPR), Protein-DNA interaction studies, Protein X-Ray crystallography, Immunoassay, Immunoelectrophoresis Enzyme Assay and inhibition studies, Protein crosslinking(formaldehyde and glutaraldehyde),.
Microbiological techniques: Growth curve of various microorganisms, various staining techniques for microorganisms & spores, Sterilization techniques, isolation of pure cultures, Biochemical Identification of Microbes engineer and manipulate microbes, Transmission electron microscopy (TEM), Phase-contrast microscopy, Antibiotic sensitivity, Metagenomics, Metatranscriptomics, MDR Identification, Enzyme Assay and other bioassays, MIC(minimum inhibitory concentration) and MBC(minimum bactericidal concentration) determination,.
Molecular Biology Techniques:, Standard and Error prone PCR (Polymerase chain reaction), qPCR, Plasmid based cloning, Cloning into bacterial expression, Restriction analysis of Plasmid DNA, Plasmid isolation in small and large scale, DNA and RNA isolation, cDNA synthesis, Agarose Gel Electrophoresis, Analysis using Gel Documentation system, Isolation of Genomic DNA, Bacterial transformation (Heat shock Method), Site directed mutagenesis, western blot , membrane stripping, SDS-PAGE, Native PAGE, Silver staining (manual and kit based),Commaisae staining and Colloidal commasiae staining.
Cell Biology Techniques: Immunoflourcence, Confocal microscopy for localization, DAPI.
Proteomics: 2D gel electrophoresis, In gel and in solution digestion of protein, Nano-LC-MS/MS, MALDI TOF/TOF, Mass spectral analysis and DATA analysis using MASCOT.
Protein Protein Interaction Techniques: Pull down assay, Yeast Two hybrid assay, Co-immunoprecipitation.
Biophysical Technique: Fluorescence anisotropy, Circular dichorisim, Spectrophotometer,spectroscopy (UV, VIS and fluorescence).
Analytical and Immunological Techniques: Western blotting, Far-Western blotting, Co-immunoprecipitation,
Immunofluorescence, Dot blot, ELISA.
Bioinformatics Techniques: Clustalw, NCBI blast, CDD analysis, Espript3.0, Phyre2, UCSF Chimera, Autodock, Phylogenetic analysis, Pymol, MD simulation.
Computer proficiency and Bioinformatics: Adobe Photoshop, ImageJ, Primer3, Espritp-3, Oligo Synthesizer, BLAST, Origin 6.0 software and Graph Pad Prism.
SUPPERVISORY AND ORGANIZATIONAL EXPERIENCE
ACADMIC ACHIEVEMENTS/AWARDS/FELLOWSHIPS
POSTER PRESENTATION/CONFERENCES
PERSONAL INFORMATION
Name: Dr. ***********
Father’s Name: Mr. ***********
Date of Birth: ***********
Gender: ****
Marital Status: ****
Nationality: Indian
Linguistic capabilities: English, Hindi, Urdu
REFERENCES
Dr. *********** Senior Scientist, India. Email: *********** Web-Page link: | Dr. ***********. Senior Scientist, India Tel: +91-xxxxxx Email: *********** Web-Page link:
| Dr. *********** Senior Scientist CDRI, Lucknow, India. Email: *********** Web-Page link: |
DELECRATION
I hereby declare that the information furnished above is relevant and correct to the best of my knowledge.
***********
Name: XXXXX
School of Chemical Engineering
xxxx University
SOUTH KOREA
TEL:+xxxxx, E-mail: xxxxxx@gmail.com
https://orcid.org/xxxxx
xxxx–xxxx | B.Sc. (Hons. xxxx), xxxx University, xxxxx, India |
xxxx–xxxx | M.Sc. (xxxxx), xxx University, xxxx, India. |
xxxx–xxxx | M.Tech. (xxxxx), xxxx University, xxxx, India. |
xxxx–xxxx | Ph.D. (xxxx), xxx, xxxxx, India. |
29 Scientific Papers and 5 Book Chapters
(Agilent SuperNova).
Father’s Name: Mr. xxxx
Mother’s Name: Mrs. xxxx
Sex: xxxx
Date of Birth: xxx
Nationality: Indian
Passport no.: xxxx
Marital Status: xxxx
Languages: English, Hindi and Urdu
Permanent Address: xxxx, India.
Professor, School of Chemical Engineering,
xxxxx University
xxxx, Republic of Korea xxxx
Email: xxxxx@gmail.com
Associate Professor, Discipline of xxxxx,
xxxx,
xxx-xxxx, India
Email: xxxxx@gmail.com
Professor, Discipline of xxxxx,
xxxxx, xxx xxxx, India
Email: xxxxxx@gmail.com
I, hereby declare that the particulars furnished by me are true to the best of my knowledge and belief.
Place: South Korea (xxxxxx)
XXX XXXX Photo
xxxxx@gmail.com
xxxx Laboratory, xxxxxx
xxxxxx, India, (+91-xxxxx)
_____________________________________________________________________
Current Position:
Senior Research Fellow, xxxxx xxxxxx xxxxxx xxxxxx India
Education:
Qualification | Subject (s) | Name of the Institution/University | Year |
PhD | Applied Science | xxxxx India | Aug, xxxx- Nov, xxx |
M.Sc | Biotechnology | Department of Biotechnology, University of xxxx | xxxx-xxxx |
B.Sc | Life sciences | xxxxxx, India | xxx-xxxx |
Publications:
Manuscript Under preparation:
Conference Proceedings:
Articles in newsprints /periodicals:
Achievements, Honors and Awards:
___________________________________________________________________
Research Experience:
Summary: Oral squamous cell carcinoma is often associated with a lymphocytic infiltrate that is believed to mount an in vivo immune reaction to the tumor cells. The extent of the lymphocytic infiltrate has been correlated with clinical prognosis, suggesting the relevance of the immune response to tumors. Accumulating evidence indicates that a dynamic cross-talk exists between tumours and the immune system that regulates tumour growth and metastasis. The focus of the present study is to identify factors secreted by oral tumor that affect key signaling events in lymphocytes and also to understand the role of myeloid-derived suppressor cells (MDSC) in impairing immunity in oral cancer patients.
xxxxxxxxx:
Part I: Extracellular xxxx mediates TCR xxxx downregulation via xxx activation in oral cancer.
Observations:
Part II: Defining the role of xxx in down regulation of xxxx cell signaling molecule xxxxx in oral cancer patients
Observations:
Part III: “Inhibition of xxxx signaling alters xxxx expression in human xxxx cells by regulating xxxx ligases.”
Observations:
xxxxxxxxx.
Part IV: Contribution of xxxx derived suppressor cells, Regulatory xxxx cells and xxxx cells in the inflammatory tumour microenvironment of oral cancer patients.
Observations:
Additional projects:
Title: “Role of xxx in regulation of xxx lymphocytes functions.”
xxxxx, xxxx and xxxx
Summary: xxx cells recognize phosphorylated antigens and play an important role during infections and in tumor immunity. The aim of the present study was to investigate the role of Notch signaling pathway in antigen specific responses of xxx cells. xxx cells were found to be predominately expressing xxx and xxx genes. The presence of active Notch intracellular domain in γδ T cells was confirmed by western blotting. Proliferation of xxx cells and IFN-γ release in response to xxx and xxxx stimulation was inhibited in the presence of GSI. Diminished lysis of tumor targets by γδ T cells as shown by 51Chromium release assay in the presence of GSI or by specifically knocking down the Notch genes, confirmed that Notch is involved in cytotoxic effector functions of xxxx cells.
Title: “xxxx”.
xxx-xxxx (MSc dissertation) xxxx University (Supervisor: Dr. xxxx)
Summary: Pxxx is an important component of apoptotic response and life span determinant. xxxx, a xxxkda protein, has been identified as a splice variant of xxxx, but unlike these variants, which are involved mainly in the transmission of mitogenic signals, xxxx functions in the intracellular pathway by converting oxidative signals into apoptosis. However, recently in human carcinomas, xxx has been found to be involved in regulating their proliferation. Elevated expression of p66shc protein is observed in prostrate carcinomas, esophageal carcinoma as well as in all kinds of proliferating thyroid tissues. But its role in human carcinogenesis has not been thoroughly dealt with. Hence, its expression in cancers needs to be thoroughly investigated which may help in understanding the signal transduction pathway operating in cancers. We extended our work on role of p66shc expression in colorectal and bronchogenic carcinomas. Our data support for a novel cancer-specific role of p66shc in colorectal and lung carcinomas
_______________________________________________________
Technical Expertise:
Field of Interest:
Innate and Adaptive Immunity, T cell differentiation and signal transduction, Tumour Microenvironment, Immune dysfunction, Inflammation, Cancer Immunology, Myeloid derived suppressor cells, Regulatory T cells, Th17 cells, Notch Signaling in T cells, Gamma Delta T cell Biology, Tumour associated Macrophages,
Presentations and participation in conferences/workshops:
Professional Association:
____________________________________________________________
Personal Details:
Date of Birth | : | xxxxx |
Gender | : | Xxxx |
Nationality | : | Indian |
Marital Status | : | xxxxxx |
Languages Known | : | English, Hindi, Urdu, xxxx, xxx |
Hobbies | : | Reading books, playing badminton and Cricket |
References:
Prof. xxxx Principal Investigator, xxxx, xxxxx -xxxxxx xxxxxx@gmail.com [PhD Supervisor] | Prof. xxxxxx Principal Investigator, xxxx, xxxxx -xxxxxx xxxxxx@gmail.com |
Prof. xxxxxx Principal Investigator, xxxx, xxxxx -xxxxxx xxxxxx@gmail.com | Dr. xxxx Principal Investigator, xxxx, xxxxx -xxxxxx xxxxxx@gmail.com |
XXXXX, Ph.D.
XXX, Leukemia Lab., XXX, India-XXXX Email Id: XXX@gmail.com, Skype- XXXX, Mobile: +91XXXX, +91XXXX
Current Position:
Professional Experience:
Educational details:
Ph.D in Life sciences (xx. xxxx- xx. xxxx)
Address of institute: XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
[Workplace] XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX, India
M.Sc. Biotechnology, First Division (XXXX-XXXX)
Address of institute:, XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
India
B.Sc. (Hons) Chemistry, First Division (XXXX-XXXX)
Address of institute:, XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
India
Publications:
Manuscript (Communicated/under preparation):
Awards and Honors:
Research experience:
xxxx XXXX- contd. (Senior Research Fellow)
Leukaemia Lab.,XXXXXXXXXXXXXXXXX.
Research project: Title
Mentor: Dr. xxxxx
Summary: Summarize project work in 2-3 lines.
xx xxxx-xx xxxxx (Postdoctoral Research Associate)
Structural & Molecular Biology Lab University of Nebraska Medical Centre, NE, USA
Mentor: Prof. xxxxx
Research project: xxxxxxxx
Summary: Cloning, expression and purification of xxx, xxx and xxx 1-4, DNA replication proteins.
xx xxxx- xx xxxx (Scientist on DBT project)
Address:, India.
Mentor: Dr. xxxxxx
Principal Investigator & Scientific Officer xxx, xxxxxxx, India.
Research project: Co-crystallization of xxxx protein with inhibitors
Summary: xxxx protein is purified with affinity chromatography followed by size exclusion chromatography. Co-crystallization was performed with phospho-peptide and small molecule inhibitors.
xx xxxx- xx xxxx, Ph.D.,
Structural & Molecular Biology Lab (xxx Lab.) xxx, India-xxx.
Mentor: Dr. xxxx xxxx
Principal Investigator & Scientific Officer, xxxxx, India.
Thesis title: “Structural and Functional Basis of xxx xxxx (xxxxx) Pathway”
Summary of Doctoral work:
Fanconi anemia (FA) is a genomic instability and cancer predisposition syndrome. FA proteins mainly xxx and xxx have pivotal role in DNA intercrosslink repair. However, structure, functions and interactions of these proteins are not well understood. Therefore, in my Ph.D Thesis, I demonstrated the structural and functional role of human xxxx repeat (cite here with published work or under communication/preparation). I investigated the structural and functional properties of xxxx domain and its biologically relevant mutants (xxxxet al. manuscript under preparation). Furthermore, I performed the interaction studies of the functional domains present in the xxx, xxxx and xxxx proteins (cite here with published work or under communication/preparation). In addition, I have also worked with my colleagues on structural and functional studies of xxx complex and xxx proteins, that involved in homologous recombination repair (cite here with published work or under communication/preparation, or list all co-authors article). Beside this, I also worked in a team to delineate the effect of novel anticancer drugs on apoptosis and cancer (cite here with published work or under communication/preparation, or list all co-authors article).
xx xxxx-xx xxx, (Collaborative work)
Molecular Biology Division, xxxxxxxx
Mentor: Dr. xxxx
Scientific officer and Head, Molecular Biology Division
Research project: Protein-protein interaction studies of xxx, xxx and xxx using bacterial two hybrid system
Summary:
Fanconi anemia pathway proteins such as xxx, xxx and xxx have conserved domains and they are known to interact with each other during DNA ICL repair. However, very little is known about the functions and interactions of the functional domains present in xxx, xxx and xxx. Therefore, I did the protein-protein interaction studies using in-vivo (bacterial two hybrid), in-vitro and in-silico approach. I also explored the effect of cancer causing mutations on the protein-protein interactions (cite here with published work or under communication/preparation).
xx xxxx-xx xxxx, (M.Sc dissertation)
Address- xxxxxx, xxxxx, India.
Mentor: Dr. xxxx
Former Deputy Director and Head, xxxxxxxxx
Dissertation title: “Genetic polymorphism of xxxxx gene in oral xxxxx fibrosis patients”.
Summary of Dissertation:
Genetic polymorphism varies with ethnicity and geographical distribution. In order to delineate the novel SNPs in the oral sub-mucous fibrosis patients, I performed RFLP and SNP in xxxx gene in the genomic DNA of the patients exclusively from xxx Indian population.
Techniques known and applied:
Primer designing, PCR, Overlap extension PCR, Molecular cloning, Site directed mutagenesis, RFLP, SNP, h-DNA isolation, Plasmid isolation, RNA isolation, guideRNA designing, CRISPR-Cas9, Human Cell line culture (OCI-AML-2/3, MCF-7, T47D, etc.), Electroporation, Transfection, Protein purification (Bacterial and yeast system), SDS-PAGE, Western Blotting, Native PAGE, Affinity Chromatography, SP/Heparin/Q/MonoQ/ResQ column purification, Size exclusion chromatography by FPLC (AKTA/BIORAD) and Hydrophobic interaction chromatography, Limited proteolysis, Protein crosslinking assay, Mass spectrometry, Peptide mass fingerprinting, EMSA by autoradiography
Circular dichroism, Fluorescence spectroscopy, Dynamic Light Scattering, Tm, Stokes radii & thermodynamic calculation, Surface Plasmon Resonance, Co-immunoprecipitation, Western blotting, Bacterial two hybrid, Beta-galactosidase assay, Pull down assay, Chaperonin like assay
Crystallization strategies, Protein Crystallization (Hanging & Sitting drop), micro-seeding and Co-crystallization, X-ray diffraction (Home source & Synchrotron Indus-2), Structure solution by MR (CCP4i and PHENIX), Molecular modelling, Molecular dynamics simulation, Principal component analysis, Protein-protein, protein-ligand and DNA-ligand Docking
Computer and other skills:
Windows and Linux based OS, Microsoft Office, Various bioinformatics tool like BLAST, FASTA, Expasy, SwissModel, Modweb, GROMACS, HEX, HADDOCK, Autodock, PYMOL, UCSF Chimera, Pubmed tools, Endnote, Mendeley, Origin, GraphPad Prism software & Adobe photoshop
Professional Skill:
Workshop and Meeting:
Conferences:
Personal Details:
Father’s Name: Dr. xxx
Mother’s Name: xxxxx
Year of Birth: xxx
Nationality: Indian
Marital Status: Single
Gender: Male
Languages Known: English, Hindi and Urdu.
Permanent Address: xxxxxxx, India-xxxxx
Referees:
Scientific Officer, Principal Investigator- xxxxxxx, Indiaxxxx
Email: xxxxxxx@gmail.com, Phone: +91-xxxxxxxx
Web-Page link: xxxxxxxx
Scientific Officer, Principal Investigator- xxxxxxx, Indiaxxxx
Email: xxxxxxx@gmail.com, Phone: +91-xxxxxxxx
Web-Page link: xxxxxxxx
Scientific Officer, Principal Investigator- xxxxxxx, Indiaxxxx
Email: xxxxxxx@gmail.com, Phone: +91-xxxxxxxx
Web-Page link: xxxxxxxx
Declaration
I do hereby declare that all the information stated above is true to the best of my knowledge and belief.
Place: xxxxx Dr. xxxxxxxx
Dr. xxxxx
Lab No.xxx, School of xxxx,
xxxxx, India
Tel: xxxxxx (M),
Email id:xxxxxx@gmail.com
To excel in my every endeavour with the inputs of hard work, focus, dedication, experience and urge to learn new technologies and explore new opportunities for their further implementation and growth, thereby contributing a step in the endless journey of development in science.
PROFESSIONAL EXPERIENCE
POSITION | PLACE | DURATION |
Post-Doctoral Fellow | Division of Biology, xxxx University, xxxx, USA | xxx December, xxx to xxxth October, xxxxx |
Post-Doctoral Fellow | Department of Biochemistry, University of xxx, xxx, USA | xxth December, xxxx to xxth May, xxxx |
Currently doing research on CRISPR/Cas:
Introduction: xxx gene is present in chromosome xx of human which codes for mitochondrial protein that regulates mitochondrial iron transport and respiration. Increase in number of xxx repeats in its intronic region leads to development of xxxx’s xxx disease which is a neurodegenerative disease. Increase in number of xxx repeats lead to decrease in xxx expression. In unaffected person, xxx expression is maximum when one of the xxx copies is affected. FXN mRNA levels decrease at some extent and when both the copies are affected, xxxx mRNA levels completely decreased. Briefly, describe about the previous work done in our lab. We used synthetic transcription elongation factor to selectively activate xxxx expression in xxxx patients. xxxx is basically composed of two elements: one is polyamide that binds to xxx repeats of xxxx gene and other is xxx which recruit elongation machinery to the site where it is bound. The crystal structure of polyamide nucleosome complex and xxx-xxxx complex designed to identify appropriate site for conjugation. After conjugating, we inserted this xxxx in xxx patient cells. The polyamide fragment in this xxx will bind to xxx repeats and attached xxx. xxx will then bind to xxx and then will recruit elongation machinery to the site and allow transcription to occur. xxxx mRNA is then transcribed. This leads to rise in xxx mRNA expression.
Intoduction: xxxx xxx is the most common inherited disease that causes progressive damage to the nervous system. This disease is caused by the genetic mutation (expansion of an intronic xxx triplet repeat in the xxx gene) leads to reduced expression of the mitochondrial protein xxx. It can also lead to scoliosis, heart disease and diabetes, but does not affect cognitive function. The disease is progressive, and ultimately a wheelchair is required for mobility. Its incidence in the general population is roughly 1 in 50,000. Here we demonstrate the creation of robust xxxx reporter or xxx reporter lymphobalstoids/Fibroblasts cell line by knocking in a Nano luciferase (Nluc) or Enhanced Green Fluroscent Protein(eGFP) gene into the human xxx gene (using CRISPR/Cas9 genome editing method. These reporter line is a good resource for drug screening as well as testing potential genetic reactivation strategies.
EDUCATIONAL BACKGROUND |
Course | Institute | Year |
Ph.D in xxx
Thesis Title: xxxx | xxxxx, (Supervisor: Professor xxxx) and xxxx University (Co-supervisor : xxxxx) | xxxx-xxx |
M.Sc in xxxx | xxxx | xxxx-xxxxx |
B.Sc (Hons) xxxxx | xxxxx | xxxx-xxx |
SELECTED PUBLICATIONS |
BOOK CHAPTERS
AWARDS AND FELLOWSHIPS |
PARTICIPATION IN CONFERENCES |
INTERNATIONAL CONFERENCES
RESEARCH EXPERIENCE:
EXPERIMENTAL SKILLS/ COMPETENCY |
PERSONAL DETAILS
|
REFERENCES
|
1.Professor xxx
School of xxx
xxx University
xxx-xxxx
India
Email id: xxxxxxx@gmail.com
2.Dr xxxxxx
Scientist
xxxxx
xxxxx
India
Emailid: xxxxxxxx@gmail.com
Vice Chancellor, xxxxxx
xxxx
xxxx
Emailid: xxxxxx@gmail.com
Director
xxxxx
xxxx
xxx, xxxx
xxxx
Emailid:xxxxx@gmail.com
Name: xxxxx
Cxxxx – xxxxx-xxxxx, INDIA
Phone: + 91-xxxxxx, Email: xxxxxx@gmail.com, Skype name: xxxxx
EDUCATION
Master of Science in xxxxx
xxxxx University, xxxx, INDIA
July xxxx
Engineering, Metabolism, Immunology, Enzymology, Biomolecules, Microbiology, Genomics, Bioprocess Engineering.
Bachelor of Science in xxxx July xxx xxxxx University, xxxx, INDIA
RESEARCH EXPERIENCE
xxxx Division, xxxxx, xxx, INDIA
Supervisor: Dr xxxxx, Scientist.
• Research Fellow in Molecular Endocrinology | xxx-xxx |
xxxxxx, INDIA |
Supervisor: Prof. xxx
• Research collaborator in xxxx | xxxxx | |
xxxxx Medical College and xxxxxx – xxxx, INDIA | ||
Research Colloborator: Dr xxxxx | ||
Zinc, copper and oxidative stress studies in xxxx positive cases. | ||
• | M.Sc Thesis student | xxxx-xx |
Protein Biology lab, xxxxx, xxxx, INDIA | ||
Supervisor: Prof. xxxx. | ||
Purification and characterization of molten globule state of xxx. | ||
(Thesis work for partial fulfillment of Master of Science in xxx) | ||
• | B.Sc Thesis Student | xxxx-xx |
Civil and Enviromental sciences lab, xxxx, INDIA
Supervisor: Ass. Prof. xxxx
(Thesis work for partial fulfillment of Bachelor of Science in xxxx)
PUBLICATIONS
RELEVANT SKILLS
Third Generation Tet-On system (pTRE3G & pCMVTet3G); Plasmid DNA Transformation; lentiviral transduction; Plasmid DNA and Si RNA Transfection; plasmid DNA isolation; Genomic DNA & RNA isolation from tissues and
Cultured cell; Restriction digestion Analysis; Electrophoresis (SDS‐PAGE & Agarose); PCR; Cloning of PCR products; site directed mutagenesis; Real time PCR; Primer Designing, Microbial culture media preparation; Culture maintenance.
Immunoblotting techniques (western blotting); Immunohistochemistry, FACS; Co-Immunoprecipitation (CO-IP), Chromatin Immunoprecipitation (ChIP), Flourescence and confocal microscopy.
Recombinant protein production; Purification of GST and His tag proteins (GST and Ni-NTA column); Spectrophotometry (UV, VIS Fluorescence).
Diabetes induction using alloxan monohydrate in swiss albino mice; orthotopic syngenic mouth cancer model development in C3H-HEJ mice; orthotopic syngenic Breast cancer model development in BALB/c mice and Sprague dawley rats; Xenografting tumors; Anesthetizing animals; organ isolation; blood collection from eye and heart.
Sub cellular fractionation, cell signaling, Media preparation; passaging; culturing; freezing of cell lines, Stable cell line preparation (Genticin/puromycin/hygromycin selection).
oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) measurements by XFe 24 Extracellular Flux Analyzer (Beginner), Comet assay, In Vivo Ubiquitination assay, In vitro Kinase assay, Luciferase assay, Killing curve assay, Sodium Rhodoamine B (SRB) Assay; MTT assay, ELISA.
Autodock (Beginner); Clustal w; Expasy; Snapgene; Oligo; NCBI nucleotide and Protein database, MS word; MS PowerPoint; MS Excel, Adobe Photoshop CS6, Mac OS, Windows OS (Windows 10, windows 8, windows 7).
AWARDS/HONOURS/ACHIEVEMENT
REFEREES
Scientist, xxxx Division
Govt of India
+91 xxxxx, xxxxxx@gmail.com
Senior Scientist, xxx Division
Govt of India
+91 xxxx, xxxxxxx@gmail.com
xxxx
xxxx lab
xxxx-xxxx, India
Email ID: xxxxx@gmail.com Mobile: +91-xxx, Lab: +91-xxx ext. xxx
SUMMARY OF QUALIFICATIONS
Ph.D. in Life Science, have studied signaling pathways leading to changes in xxx in human xxx cancer and DNA damage response using genetic manipulation and biochemical approaches, and, have also analyzed the utility of xxx inhibitors in combinatorial chemotherapy by studying cell physiology, chromatin organization and drug kinetics. Have experience in generating stable clones of bacterial proteins to understand their functions in pathogenicity and/ or tumor development.
EDUCATION
Ph.D in Life Sciences, xxx, India. | xxx-Present | ||
M.Tech in Biotechnology, xxxx, xxxx, India. | xxxx-xxx | ||
M.Sc in Biotechnology, xxxx, xxx, India. | xxx-xxx | ||
B.Sc in xx, Botany and xx, xxxx xxx, India. | xxx-xx | ||
RESEARCH PUBLICATIONS | |||
Under review / To be submitted / Under preparation: | |||
Published / Accepted:
RESEARCH EXPERIENCE
Project title: xxxxxx
Enzymes. Mentor: Dr. xxxxx
Objectives of the study:
Outcomes of the study:
Dissertation title: | Study of comma shape of xxx and its strain specific difference in pathogenicity and | ||
xxxx activity. | Mentor: Dr. xxxxxx | ||
Objectives of the study:
Outcome of the study:
Using homologous recombination endogenous copies of xxxx elements (xxxx) and enzymes of xxxx synthesis pathway (xxxx) replaced and overexpressed in xxx strain of xxxx and shape defects were observed with electron microscopy. Also, xxx showed more pH tolerance compared to xxx xxxx which is dependent of their intrinsic xxxx activity.
SKILL andEXPERTISE
Biochemical and Molecular Biology Techniques: SDS-PAGE, AUT-PAGE, 2D SDS-AUT PAGE, Variants of PCR, RT-PCR and Molecular Cloning.
Cell Culture and Cell Biology Techniques: Handling mammalian cell cultures, Transfection, Cell proliferation and cytotoxicity assay (MTT, Crystal Violet, and SRB), Clonogenicity assay, Microscopy (Confocal, Electron) and Flow cytometry.
Analytical and Immunological Techniques: Western blotting, AUT-Western blotting, Co-immunoprecipitation, Immunofluorescence, Immunohistochemistry, Spectrophotometry (UV, VIS and fluorescence), MALDI-TOF and Micrococcal nuclease assay.
Experiments on Human Tissue:Collection and storage, Microtome sectioning and Immunohistochemistry on paraffin imbedded and frozen sections, Chromatin and cytoplasmic fractionation, DNA and RNA isolation and Maintenance of clinical data.
Experiments on Animal Model: Xenograft tumor models (in Nude and NOD-SCID mice).
Computer proficiency and Bioinformatics: Adobe Photoshop, ImageJ, LSM-10, DNASTAR, Primer3, Oligo Synthesizer, BLAST, SPSS (Statistical Package for the Social Sciences) and GraphPad Prism.
SUPPERVISORY and ORGANIZATIONAL EXPERIENCE
ACADMIC ACHIEVEMENTS/AWARDS/FELLOWSHIPS
CONFERENCE PRESENTATIONS
REFERENCES
Dr. xxxxx | Dr. xxxxx | Dr. xxxxx |
Scientific Officer | Scientific Officer | Scientific Officer |
xxx | xxx | xxx |
xxxx | xxxx | xxxx |
xxxx | xxxx | xxxx |
Email: xxxxxx@gmail.com | Email: xxxxxx@gmail.com | Email: xxxxxx@gmail.com |
Name: XXXXX
Address: XXX, XXXXXXXXXXX, City (State) – Pin code
Contact No.: +91-xxxxxxxxxxx, Email ID: xxxxxxxx@gmail.com
WORK EXPERIENCE
Senior Consultant (Research) – Smart Cities Project (December xx till date):
Team Lead – Business Loans Group (BLG), Raipur (July xxxx – May xxxx):
Relationship Manager – Business Loans Group (BLG), Raipur (June xxxx – April xxxx):
INTERNSHIPS and TRAININGS
EDUCATION
ADDITIONAL ACTIVITIES
ResearchTweet acts as a Catalyst on enzyme kinetics platform (Research Job Search). It lowers activation energy (easier, faster, focused) of Substrates (YOU) struggling to land into Products (Jobs).
Share
Looking For Postdoctoral Position?