PhD project in Molecular basis and ecological significance of Saccharomyces wine yeast interactions with microbes, plants and insects

This research involving Saccharomyces cerevisiae will train you with skills you can apply to other ‘yeast’ labs or biological systems.

Saccharomyces was the first sequenced eukaryote and comes with many indispensable tools for exploring genetic and subcellular mechanisms. This yeast is also used in the multi-billion-dollar fermentation industries and is found in nature.

Despite its importance, the significance of elements of yeast’s metabolism, its natural ecology and interactions with other microbes, plants and insects isn’t fully understood. For example, yeast produce volatiles that we find pleasing in fermented products such as wine, but this production probably evolved for another purpose, not our benefit.

It seems to have evolved to perform either of these functions:

• attract insects (e.g., the vinegar fly, Drosophila melanogaster) to vector the non-motile yeast to fresh nutrient-rich niches and/or
• signal the switch from the planktonic yeast cell state to forming chains that spread across or invade solid growth media to ‘forage’ for nutrients

This study seeks to:

• identify new yeast signaling mechanisms
• identify the precise triggers for the switch in yeast cell morphology
• see how different morphologies aid survival, vectoring and competition in mixed microbial populations

The project will involve diverse techniques including field isolation of microbes and their genetic modification, transcriptomics, bioinformatics, microscopy and analytical chemistry (GC-MS, HPLC). The findings will provide insight into fundamental processes of Saccharomyces and its pathogenic relatives along with likely applications in the multi-billion-dollar fermentation industries and development of bioprotection treatments in food and agriculture.

Entry requirements