The Moseman Laboratory at Duke University is seeking a detail-oriented individual for the position of Lab Research Analyst II to join our team. The Moseman Lab uses a variety of infectious pathogens together with transgenic mouse models, flow cytometry, confocal and intravital imaging, and transcriptomic analyses to study host:pathogen interactions within the upper airway and brain. Read more about our interests at https://www.mosemanlab.com/
Responsibilities
- Manage a transgenic mouse colony, including but not limited to: live mouse handling, ordering, genotyping, breeding, routine monitoring of animal health, sample collection and processing, maintaining and updating mouse line records etc.
- Assist in the design and analysis of experiments; carry out experiments with minimal or no immediate supervision and record and maintain all acquired data in specified formats for scientific reliability and general comprehension
- Administering compounds to mice (injections, oral gavage), performing dissections to isolate and characterize lymphocytes from various tissues, and conducting relevant animal models of disease (e.g., infections and immunizations)
- Process mouse biospecimens such as blood and tissues for flow cytometry, histology, viral and antibody titering, DNA, RNA, etc. Perform simple techniques in cell biology and microscopy: examples include cell isolation and culture procedures, immunocytochemistry, tissue cryosectioning, microscope imaging, cell sorting, and some basic molecular biology and biochemistry.
- Maintain cell culture lines, including preparation of culture media, chemicals, reagents and solutions
- Perform routine lab maintenance, cleaning, ordering, and organizing.
- Maintain lab SOPs, maintain organization of the lab which includes all equipment, freezers, fridges, reagents in these areas and general upkeep of lab space.
- Understand, comply and assist in enforcing all safety standards and procedures to ensure a safe working environment for all laboratory staff
- Maintain adequate stock of lab supplies and ensure proper setup and functioning of equipment.
- Maintain detailed and accurate documentation of activities in an electronic laboratory notebook.
- Learn and develop new lab techniques and assist in the training of new personnel.
- Employee may be directed to perform job-related tasks other than those specifically presented in this description.
Preferred Skills:
- Excellent interpersonal, problem-solving, communication and organizational skills.
- Experience with the care and handling of mice, flow cytometry, and molecular biology techniques is preferred, ideally with some experience outside of laboratory classwork (i.e. in a working laboratory)
- Analytical skills to resolve complex problems requiring the use of scientific, mathematical, or technical principles and in-depth, experienced-based knowledge
- Flexibility, motivation, and ability to work independently.
- Willingness and ability to take on multiple projects simultaneously.
Minimum Qualifications
Education
Work requires a bachelor’s degree in botany, biology, zoology, psychology or other directly related scientific field.
Experience
Work requires four years of research experience. A related master’s degree may offset required years of experience on a 1:1 basis, e.g., a two year master’s degree in lieu of two years of experience.
