A postdoctoral position is available in Dr. Lixin Wan’s group at Moffitt Cancer Center Department of Molecular Oncology.
The research in Dr. Wan’s lab is focused on protein post-translational modifications, including ubiquitination, acetylation, methylation, and phosphorylation. We are interested in understanding how these modifications are misregulated in tumor cells, tumor-infiltrating immune cells, and tumor-interacting stroma cells. We are also interested in developing next-generation techniques to characterize these modifications. Our current projects include the multifaceted roles of ITCH in melanoma and the melanoma immune microenvironment, the context-dependent functions of master cell cycle regulator APC/C in breast cancer and ovarian cancer, the crosstalk between tumor cell aneuploidy and innate immunity, and several others.
The lab uses a wide range of approaches in our projects: molecular biology, protein biochemistry, in vitro cell culture models, xenograft and syngeneic mouse models, genetically engineered mouse models, various CRISPR-based techniques, and genomic/transcriptomic/proteomic omics techniques. Our recent representative publications: Qing et al, Nature Communications 2019; Han et al. Nature Communications 2019; Dai et al. Cell Reports 2022. Others can be found on Dr. Wan’s Google Scholar page: https://scholar.google.com/citations?user=VKWLR3MAAAAJ
The Ideal Candidate:
- Experienced in molecular biology, cell biology and biochemistry techniques including cloning, virus delivery system, CRISPR/Cas9 system, DNA/RNA manipulation, protein purification, and immunoblot.
- Animal handling, mouse modeling and small animal imaging experience.
- Basic imaging techniques including immunofluorescence, live color imaging.
- Basic bioinformatics skills.
- Previous experience in next-generation sequencing techniques (RNA-seq, ChIP-seq, etc) and/or proteomics is a plus.
- Previous immunology experience is a plus.
Responsibilities:
- Work with PI on the project planning; design and conduct experiments independently; document and organize the data routinely.
- Learn and develop research techniques and lab protocols.
- Develop scientific writing and presentation skills.
- Work with collaborators and core facility staff on interdisciplinary experiments.
- Supervise research assistants and graduate students.
Credentials and Qualifications:
- MD or PhD in Cancer Biology, Molecular and Cellular Biology, Genetics or related.
Interested applicants should send a single PDF file that includes a cover letter summarizing their research training and accomplishments, a personal statement of scientific interests and goals, current CV with recent publications, and contact information for three references to Dr. Lixin Wan (Lixin.Wan@moffitt.org).
