The Nolan lab is housed in the College of Veterinary Medicine (CVM) at NC State University (Raleigh, North Carolina). Dr. Nolan runs an active research program primarily focused on the intersection of neurobiology and cancer/radiation biology. Our long-term goal is to identify novel targets for improved clinical management of pain that results from cancer treatment. We are also interested in understanding how pain signaling can influence the progression of cancer. To accomplish these goals, we utilize a combination of in vitro (cell culture) and in vivo (rodent/mouse) models, as well as clinical trials in both veterinary and human cancer patients.
- Laboratory Work:
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- Conduct mammalian cell culture, histology, microscopy (including independent execution of immunohistochemistry and slide digitization), and molecular biology techniques (WB, ELISA, qRT-PCR, genotyping, transfection).
- Analyze and prepare data, contributing to the interpretation of results and determining the next steps for ongoing projects.
- Manage multiple concurrent projects, ensuring timely and accurate completion.
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- Animal Work:
- Perform basic mouse handling, behavioral testing, injections, and anesthesia.
- Provide research support, including interaction with laboratory animal staff and ensuring adherence to animal care protocols.
- Assist in the preparation of animal care and use protocols.
- Collaboration and Training:
- Train and mentor undergraduate and graduate students, and collaborate with postdocs and other lab members on experimental procedures.
- Interface with outside collaborators and clinical staff, particularly within our small animal veterinary hospital, with potential involvement in veterinary clinical trials.
- Documentation and Literature Review:
- Maintain detailed records of all completed tasks and experimental procedures.
- Stay informed on relevant literature and research developments, applying this knowledge to ongoing projects.
- Manage the scheduling of laboratory procedures.
Other Responsibilities
The position will troubleshoot lab-based problems and will assume some responsibilities for the overall running of the lab, including training of laboratory staff and students. Up to 10-20% of the position’s time may be dedicated to laboratory management activities, which include ordering and maintenance of laboratory supplies and equipment (e.g., inventory, purchasing, coordination with service engineers); updating laboratory safety plans; maintaining stock solutions; autoclaving, etc.
Minimum Education and Experience
An undergraduate degree (e.g., B.S.) with 3 years of experience in animal handling and molecular biology.
Other Required Qualifications
- Prior research experience including mammalian cell culture, basic histology and microscopy, and molecular biology techniques (WB, ELISA, qRTPCR, genotyping, transfection).
- Strong verbal and written communication skills. Excellent organization skills with strong attention to detail.
- Ability to work to tight deadlines, goal-oriented, and high level of motivation.
- Ability to work individually and also as part of a team.
- Competency with Microsoft Office (e.g., Word, Excel). Strong fine motor skills.
- Must be able to lift up to 30 pounds.
- Prior rodent-based research experience (including mouse colony management {breeding, genotyping, etc.} and basic mouse handling, injections, and anesthesia)
