Electron microscopes are extravagant to assemble and keep up with, yet the funds and running expenses of confocal light microscope system currently covers with those of fundamental electron microscopes.
Microscopes intended to accomplish high resolutions should be housed in stable structures with uncommon administrations, for example, magnetic field cancelling systems.
The examples to a great extent must be seen in vacuum, as the molecules that make up air would scatter the electrons.
An exemption is liquid-phase electron microscopy utilizing either a closed liquid cell or environmental chamber, for instance, in the environmental scanning electron microscope, which permits hydrated examples to be seen in a low-pressure (up to 20 Torr or 2.7 kPa) wet environment.
For in situ electron microscopy of different techniques, gaseous samples have also been created.
SEM working in traditional high-vacuum mode normally image conductive specimens; in this way non-conductive materials require conductive covering (gold/palladium combination, carbon, osmium, and so forth).
It becomes possible to observe a non-conductive material without coating using the low-voltage mode of modern microscopes.
Minute, stable specimens like carbon nanotubes, diatom frustules and little mineral crystals (asbestos filaments, for instance) doesn’t require any special treatment prior being observed in the electron microscope.
Samples of hydrated materials, including practically all biological specimens must be ready in different manners to balance out them, diminish their thickness (ultrathin sectioning) and increases their electron optical contrast (staining).
These cycles might bring about artefacts, however these can for the most part be recognized by contrasting the outcomes got by utilizing drastically unique specimen arrangement methods.
Since the 1980s, examination of cryofixed, vitrified specimens has additionally gotten progressively utilized by researchers, further affirming the legitimacy of this technique.