In vitro Cell Migration, Wound Healing Full Protocol
What is Cell Migration? Cell migration, movement of cells from one area to another generally in response to a chemical stimulus. Migration is a central to achieving functions such as…
What is Cell Migration? Cell migration, movement of cells from one area to another generally in response to a chemical stimulus. Migration is a central to achieving functions such as…
What is Cell Culture? Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favourable artificial environment. The cells may be…
About Immunocytochemistry Immunocytochemistry (ICC) is a common laboratory technique that uses antibodies that target specific peptides or protein antigens in the cell via specific epitopes. The antibodies bound on diffirent…
About Calcein AM / PI Cell Viability Assays The Calcein –AM/PI assay is a two-colour fluorescence cell death assay that is based on the simultaneous determination of live and dead…
What is Electroporation? The process of introducing nucleic acids (DNA or RNA) into eukaryotic cells by nonviral methods is defined as transfection. Using various chemical, phospho-lipid or physical methods, this…
About Apoptosis Annexin V is a phospholipid binding protein with high affinity for binding to phosphotidyl serine (PS), which is a phospholipid present exclusively in the inner leaflet of the…
About Neutral Red Cell Viability Assay The NRU cytotoxicity assay or Neutral red uptake assay procedure is a cell survival or cell viability chemosensitivity assay based on the ability of…
"Main challenge with the surgery is the recurrence of the tumor from the cells which are left during surgery there should not be a single tumor cells left during surgery"…
Gel Electrophoresis Objective To perform SDS-PAGE Gel Electrophoresis for separation of proteins. About Gel Electrophoresis Almost most of the analytical electrophoresis of proteins is carried out in polyacrylamide gels under…
About Thawing of Frozen Cell Cells that are cryopreserved can be used again whenever required. The procedure of bringing frozen cells to room temperature from a metabolically inactive state to…